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deoxynucleotide dntp solution mix  (New England Biolabs)


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    New England Biolabs deoxynucleotide dntp solution mix
    Deoxynucleotide Dntp Solution Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2095 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deoxynucleotide+solution/Deoxynucleotide+(dNTP)+Solution+Mix/pmc13010419-9-0-5
    Average 99 stars, based on 2095 article reviews
    deoxynucleotide dntp solution mix - by Bioz Stars, 2026-09
    99/100 stars

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    Amplification:

    Article Title: An integrated microfluidic detection system for the automated and rapid diagnosis of high-risk human papillomavirus.
    Article Snippet: Human papillomavirus (HPV) causes the prevalent sexually transmitted infection that accounts for the majority of cervical cancer incidences.. Therefore, the development of a rapid, accurate, automatic and affordable nucleic acid detection strategy is urgently required for HPV tests, among which microfluidic chip is a promising diagnostic method.. In this work, we developed a microfluidic detection system consisting of a microfluidic chip and the corresponding detection equipment to diagnose high-risk HPV.

    Article Title: Protein secretion zones during overexpression of amylase within the Gram-positive cell wall
    Article Snippet: The fusion of SecA and SecDF to mNeonGreen was cloned into the pSG1164 vector containing a sequence encoding monomeric NeonGreen [ ] and a flexible 14-amino acid linker (GPGLSGLGGGGGSL). .. For this purpose, at least 500 bp of the 3`end of the desired gene (excluding the stop codon) was amplified by polymerase-chain reaction (PCR) using B. subtilis PY79 gDNA as template, oligonucleotides (Additional file : Table S2), Phusion DNA polymerase, and deoxynucleotide solution (both from New England Biolabs, NEB). .. The resulting PCR product was integrated into the plasmid via the Gibson Assembly cloning system (New England Biolabs-NEB).

    Article Title: Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm
    Article Snippet: .. For this purpose, monomeric Venus (mV) coding sequence carrying the A206K mutation [ ] was amplified by polymerase-chain reaction (PCR) using a lab stock plasmid carrying mV as a template, Oligonucleotides Fw linker-mVenus ApaI and Rev mVenus SpeI (Additional file : Table S7), Phusion DNA polymerase, and Deoxynucleotide solution (both from New England Biolabs, NEB). .. The resulting 771 bp PCR product was digested successively with ApaI and SpeI-HF yielding a 755 bp DNA fragment with cohesive 3′ overhanging ends; the vector pSG1164 (4.8 Kbp) was digested accordingly and subsequently dephosphorylated by Calf intestine phosphatase (CIP, NEB); both linear DNA fragments were purified using agarose gel-electrophoresis followed by gel extraction (QIAquick Gel Extraction Kit, QIAGEN), ligated by T4 DNA ligase (NEB), and heat-shock transformed into chemical competent E. coli XL-1 Blue (Stratagene) to yield a strain carrying pSG1164-linker-mVenus as a plasmid.

    Article Title: Protein secretion zones during overexpression of amylase within the Gram-positive cell wall.
    Article Snippet: The fusion of SecA and SecDF to mNeonGreen was cloned into the pSG1164 vector containing a sequence encoding monomeric NeonGreen [69] and a flexible 14-amino acid linker (GPGLSGLGGGGGSL). .. For this purpose, at least 500 bp of the 3`end of the desired gene (excluding the stop codon) was amplified by polymerasechain reaction (PCR) using B. subtilis PY79 gDNA as template, oligonucleotides (Additional file 8: Table S2), Phusion DNA polymerase, and deoxynucleotide solution (both from New England Biolabs, NEB). ..

    Article Title: Protein secretion zones within the Gram-positive cell wall
    Article Snippet: The fusion of SecA and SecDF to mNeonGreen were cloned into the pSG1164 vector containing a sequence encoding monomeric NeonGreen and a flexible 14-amino acid linker (GPGLSGLGGGGGSL). .. For this purpose, at least 500 bp of the 3‵ end of the desired gene (excluding the stop codon) was amplified by polymerase-chain reaction (PCR) using B. subtilis PY79 gDNA as template, oligonucleotides (Supplementary Table S2), Phusion DNA polymerase, and deoxynucleotide solution (both from New England Biolabs, NEB). .. The resulting PCR product was integrated into the plasmid via the Gibson Assembly cloning system (New England Biolabs-NEB).

    Article Title: Full integration of nucleic acid extraction and detection into a centrifugal microfluidic chip employing chitosan-modified microspheres.
    Article Snippet: Microfluidic devices especially centrifugal ones have attracted great attention in the nucleic acid testing field, due to their automation, high efficiency, and simple operation.. In which, nucleic acid extraction is the basic step, laying a foundation for the downstream amplification and detection procedures.. Therefore, the integration of nucleic acid extraction on the chip is expected to achieve cost-efficiency, high-speed automation, diagnostic accuracy, and reaction robustness with the respect to real-time detection.

    Polymerase Chain Reaction:

    Article Title: Protein secretion zones during overexpression of amylase within the Gram-positive cell wall
    Article Snippet: The fusion of SecA and SecDF to mNeonGreen was cloned into the pSG1164 vector containing a sequence encoding monomeric NeonGreen [ ] and a flexible 14-amino acid linker (GPGLSGLGGGGGSL). .. For this purpose, at least 500 bp of the 3`end of the desired gene (excluding the stop codon) was amplified by polymerase-chain reaction (PCR) using B. subtilis PY79 gDNA as template, oligonucleotides (Additional file : Table S2), Phusion DNA polymerase, and deoxynucleotide solution (both from New England Biolabs, NEB). .. The resulting PCR product was integrated into the plasmid via the Gibson Assembly cloning system (New England Biolabs-NEB).

    Article Title: Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm
    Article Snippet: .. For this purpose, monomeric Venus (mV) coding sequence carrying the A206K mutation [ ] was amplified by polymerase-chain reaction (PCR) using a lab stock plasmid carrying mV as a template, Oligonucleotides Fw linker-mVenus ApaI and Rev mVenus SpeI (Additional file : Table S7), Phusion DNA polymerase, and Deoxynucleotide solution (both from New England Biolabs, NEB). .. The resulting 771 bp PCR product was digested successively with ApaI and SpeI-HF yielding a 755 bp DNA fragment with cohesive 3′ overhanging ends; the vector pSG1164 (4.8 Kbp) was digested accordingly and subsequently dephosphorylated by Calf intestine phosphatase (CIP, NEB); both linear DNA fragments were purified using agarose gel-electrophoresis followed by gel extraction (QIAquick Gel Extraction Kit, QIAGEN), ligated by T4 DNA ligase (NEB), and heat-shock transformed into chemical competent E. coli XL-1 Blue (Stratagene) to yield a strain carrying pSG1164-linker-mVenus as a plasmid.

    Article Title: Protein secretion zones during overexpression of amylase within the Gram-positive cell wall.
    Article Snippet: The fusion of SecA and SecDF to mNeonGreen was cloned into the pSG1164 vector containing a sequence encoding monomeric NeonGreen [69] and a flexible 14-amino acid linker (GPGLSGLGGGGGSL). .. For this purpose, at least 500 bp of the 3`end of the desired gene (excluding the stop codon) was amplified by polymerasechain reaction (PCR) using B. subtilis PY79 gDNA as template, oligonucleotides (Additional file 8: Table S2), Phusion DNA polymerase, and deoxynucleotide solution (both from New England Biolabs, NEB). ..

    Article Title: Protein secretion zones within the Gram-positive cell wall
    Article Snippet: The fusion of SecA and SecDF to mNeonGreen were cloned into the pSG1164 vector containing a sequence encoding monomeric NeonGreen and a flexible 14-amino acid linker (GPGLSGLGGGGGSL). .. For this purpose, at least 500 bp of the 3‵ end of the desired gene (excluding the stop codon) was amplified by polymerase-chain reaction (PCR) using B. subtilis PY79 gDNA as template, oligonucleotides (Supplementary Table S2), Phusion DNA polymerase, and deoxynucleotide solution (both from New England Biolabs, NEB). .. The resulting PCR product was integrated into the plasmid via the Gibson Assembly cloning system (New England Biolabs-NEB).

    Sequencing:

    Article Title: Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm
    Article Snippet: .. For this purpose, monomeric Venus (mV) coding sequence carrying the A206K mutation [ ] was amplified by polymerase-chain reaction (PCR) using a lab stock plasmid carrying mV as a template, Oligonucleotides Fw linker-mVenus ApaI and Rev mVenus SpeI (Additional file : Table S7), Phusion DNA polymerase, and Deoxynucleotide solution (both from New England Biolabs, NEB). .. The resulting 771 bp PCR product was digested successively with ApaI and SpeI-HF yielding a 755 bp DNA fragment with cohesive 3′ overhanging ends; the vector pSG1164 (4.8 Kbp) was digested accordingly and subsequently dephosphorylated by Calf intestine phosphatase (CIP, NEB); both linear DNA fragments were purified using agarose gel-electrophoresis followed by gel extraction (QIAquick Gel Extraction Kit, QIAGEN), ligated by T4 DNA ligase (NEB), and heat-shock transformed into chemical competent E. coli XL-1 Blue (Stratagene) to yield a strain carrying pSG1164-linker-mVenus as a plasmid.

    Mutagenesis:

    Article Title: Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm
    Article Snippet: .. For this purpose, monomeric Venus (mV) coding sequence carrying the A206K mutation [ ] was amplified by polymerase-chain reaction (PCR) using a lab stock plasmid carrying mV as a template, Oligonucleotides Fw linker-mVenus ApaI and Rev mVenus SpeI (Additional file : Table S7), Phusion DNA polymerase, and Deoxynucleotide solution (both from New England Biolabs, NEB). .. The resulting 771 bp PCR product was digested successively with ApaI and SpeI-HF yielding a 755 bp DNA fragment with cohesive 3′ overhanging ends; the vector pSG1164 (4.8 Kbp) was digested accordingly and subsequently dephosphorylated by Calf intestine phosphatase (CIP, NEB); both linear DNA fragments were purified using agarose gel-electrophoresis followed by gel extraction (QIAquick Gel Extraction Kit, QIAGEN), ligated by T4 DNA ligase (NEB), and heat-shock transformed into chemical competent E. coli XL-1 Blue (Stratagene) to yield a strain carrying pSG1164-linker-mVenus as a plasmid.

    Plasmid Preparation:

    Article Title: Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm
    Article Snippet: .. For this purpose, monomeric Venus (mV) coding sequence carrying the A206K mutation [ ] was amplified by polymerase-chain reaction (PCR) using a lab stock plasmid carrying mV as a template, Oligonucleotides Fw linker-mVenus ApaI and Rev mVenus SpeI (Additional file : Table S7), Phusion DNA polymerase, and Deoxynucleotide solution (both from New England Biolabs, NEB). .. The resulting 771 bp PCR product was digested successively with ApaI and SpeI-HF yielding a 755 bp DNA fragment with cohesive 3′ overhanging ends; the vector pSG1164 (4.8 Kbp) was digested accordingly and subsequently dephosphorylated by Calf intestine phosphatase (CIP, NEB); both linear DNA fragments were purified using agarose gel-electrophoresis followed by gel extraction (QIAquick Gel Extraction Kit, QIAGEN), ligated by T4 DNA ligase (NEB), and heat-shock transformed into chemical competent E. coli XL-1 Blue (Stratagene) to yield a strain carrying pSG1164-linker-mVenus as a plasmid.

    Reverse Transcription:

    Article Title: Multiplexed detection of respiratory viral pathogens by isothermal amplification on an autonomously loaded chip at the point-of-care.
    Article Snippet: All primers listed in SI Table S1 were obtained from Integrated DNA Technologies (IDT, Leuven, Belgium), diluted in nucleasefree water, and stored at −20 °C. .. WarmStart LAMP kit (DNA&RNA, E1700S), WarmStart RTx reverse transcriptase (M0380L, 15000 U mL−1), WarmStart Bst 2.0 polymerase (M0538S, 8000 U mL−1), LAMP fluorescent dye (Cat. # B1700), recombinant albumin (Cat. # B9200) and deoxynucleotide solution (dNTP, N0447S, 10.0 mM) were obtained from New England Biolabs (Ipswich, MA, USA). .. TaqMan Control Genomic DNA (Human, 10 ng μL−1) and betaine (Cat. # J77507) were obtained from Thermo Fisher Scientific (Vilnius, Lithuania).

    Recombinant:

    Article Title: Multiplexed detection of respiratory viral pathogens by isothermal amplification on an autonomously loaded chip at the point-of-care.
    Article Snippet: All primers listed in SI Table S1 were obtained from Integrated DNA Technologies (IDT, Leuven, Belgium), diluted in nucleasefree water, and stored at −20 °C. .. WarmStart LAMP kit (DNA&RNA, E1700S), WarmStart RTx reverse transcriptase (M0380L, 15000 U mL−1), WarmStart Bst 2.0 polymerase (M0538S, 8000 U mL−1), LAMP fluorescent dye (Cat. # B1700), recombinant albumin (Cat. # B9200) and deoxynucleotide solution (dNTP, N0447S, 10.0 mM) were obtained from New England Biolabs (Ipswich, MA, USA). .. TaqMan Control Genomic DNA (Human, 10 ng μL−1) and betaine (Cat. # J77507) were obtained from Thermo Fisher Scientific (Vilnius, Lithuania).



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